Mites in the genus Tropilaelaps could follow in the footsteps of Varroa mites as significant threats to honey bees if they were to expand their range. A new test using analysis of the “melt curves” of DNA samples can discern the four known Tropilaelaps species and could be an important tool in surveillance for the mite pests in apicultural settings. A technique known as high-resolution melting analysis can be used on DNA samples amplified via polymerase chain reaction. The analysis measures the temperature at which the two strands of double-stranded DNA denatures, or “melts” apart, during the test. The charts shown here display the melt curves for the four Tropilaelaps species found in a new study by researchers at ANSES, the French Agency for Food, Environmental and Occupational Health & Safety. (Image originally published in Del Cont et al 2021, Journal of Economic Entomology)