A diagram illustrates the experiment design in a research study exploring social apoptosis behavior in varying strains of honey bees (Apis mellifera): “"”For each experimental replicate, a frame from each stock containing newly laid eggs was placed in a single host colony with either a high or low Varroa mite population. Frames of brood were removed from the host colonies within 12 hr of capping of the cells to receive treatment. Cell caps were carefully removed, and brood was assigned to either control, experimental wounding, or experimental infestation with a foundress mite collected from several mite source colonies. In brood reared in highly infested colonies, we also included cells which contained a mite when opened in a fourth treatment group: naturally infested cells. Cells were then sealed with a clear gelatin cap, which could be used as a viewing window for the remainder of the study. Finally, brood was allowed to mature in an incubator to prevent removal by workers.”"” (Image originally published in Ihle et al 2022, Journal of Insect Science)