On a first-instar larvae of a honey bee (Apis mellifera), still covered in the serosa from its egg, locations of spiracles are noted in circles 1, 2, and 3 in the image at top, though the nearby hatching spines are not visible in this view. Zooming in closer (middle), the spines can be seen, including their location near the spiracle, seen in the lower-left corner of this view. At bottom, a fully zoomed-in view shows a single hatching spine with shreds of the serosa, “strongly suggesting that spines are responsible for shredding of serosa and involved with dissolution of chorion,” the authors write.